Composite

Part:BBa_K2859002

Designed by: Kyuhee Jo   Group: iGEM18_HAFS   (2018-10-10)


Insulin secretion (weak)

This part secretes Winsulin, single chain insulin, associated with penetratin and FLAG tag that allows easy detection with western blot. The coding sequence is regulated by promoter J23116

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None